In-vitro Culture and Plant Sanitation
In-vitro sanitation of viroids such as HLVd.
Exclusive in-vitro protocols for viroid sanitation (HLVd), recovery of pathogen-free mother plants, and long-term varietal preservation.
In-vitro plant services and sanitation protocols against phytopathogens.
Eliminating persistent viroids like HLVd through exclusive protocols, along with micropropagation, embryo rescue, and varietal maintenance in vitro.
At Leafgene Solutions, we apply advanced in-vitro techniques to recover and protect high-value crops. Our exclusive protocols eliminate persistent viroids such as HLVd, ensuring pathogen-free mother plants ready for propagation. We also offer large-scale clonal micropropagation, varietal conservation, seed germination, and embryo rescue, safeguarding genetic continuity and plant health. With a scientific and scalable approach, we help growers, nurseries, and cooperatives secure quality and competitiveness in the agricultural market.
In-vitro plant sanitation
The elimination of pathogens and viroids (e.g., Hop Latent Viroid, HLVd) is achieved through advanced in-vitro meristem culture techniques. This procedure involves the extraction and cultivation of apical meristems, plant tissue regions in constant cell division that are naturally free, or carry lower loads, of infectious agents.Through this approach, it is possible to regenerate complete, healthy plants, genetically identical to the mother plant but free of viroids, viruses, and other pathogens that compromise crop productivity and quality.Our know-how in in-vitro plant biotechnology, developed and optimised over years of experience, allows us to guarantee infection-free plant material with high standards of viability, genetic uniformity, and phytosanitary quality.Micropropagation
Micropropagation is a plant biotechnology technique that enables the large-scale clonal reproduction of plants through in-vitro culture. From small fragments of plant tissue —such as meristems, buds, or selected explants— it is possible to induce the regeneration of complete individuals under controlled conditions of sterility, light, temperature, and nutrients.This method ensures that each regenerated plant is genetically identical to the mother plant, preserving all of its agronomic, morphological, and productive traits. In this way, micropropagation enables the mass production of high-quality, uniform plants, which is essential for commercial plantations, breeding programmes, and germplasm conservation.In-vitro varietal maintenance
In-vitro varietal maintenance consists of the conservation and safeguarding of plant tissues under sterile, controlled laboratory conditions, ensuring the genetic and phytosanitary integrity of valuable varieties over time. Through this technique, explants are preserved in specific nutrient media and in a contaminant-free environment, thus preventing the loss of valuable plant material due to infections, genetic degradation, or adverse environmental factors.This procedure is fundamental to preserve the reproductive and productive potential of varieties, ensuring that, when required, they can be used in micropropagation processes to generate complete, uniform, and pathogen-free plants.Seed germination testing
Seed germination testing is a procedure used to evaluate the viability, vigor, and developmental capacity of seeds under controlled conditions. Through in-vitro culture techniques in a reduced space, it is possible to induce and monitor seedling formation, providing key information on the physiological and sanitary quality of seed lots.This process not only detects germination issues related to low viability, dormancy, or pathogen presence, but also ensures the optimisation of planting material, reducing risks during crop establishment. Conducted in a sterile and controlled environment, germination testing delivers fast, reliable, and reproducible results.Embryo rescue
Embryo rescue is an in-vitro culture technique that enables the extraction, isolation, and development of embryos from non-viable seeds or those with low natural germination potential. Through this procedure, embryos are cultured in sterile nutrient media under controlled conditions, promoting their survival, regeneration, and subsequent growth into healthy, viable adult plants.This methodology is particularly useful in cases involving reproductive barriers, such as interspecific or intergeneric hybrids, where the seed cannot fully develop under natural conditions.Connect with Our Experts
Discover how our advanced biotechnology solutions can protect and transform your high-value crops.
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What are the advantages of in-vitro plant sanitation?
Our micropropagation and sanitation protocols eliminate persistent pathogens such as viroids and viruses that are difficult to control. The result is clean, certifiable mother plants ready for safe propagation, reducing risk and loss.
Do you offer diagnosis and remediation for Hop Latent Virus (HLVd) in cannabis and hops?
Yes. HLVd is one of the main problems in cannabis and hops, with yield losses exceeding 30%. At Leafgene Solutions, we offer:
- High-precision molecular diagnostics to detect infections in mother plants and nurseries. We are capable of applying different techniques to adapt to your specific needs: single plants or batches of plants, one-time or recurring.
- Exclusive in vitro plant sanitation to recover viroid-free material and establish certified mother plants.
¿Qué servicios principales ofrece Leafgene Solutions?
Nuestra actividad se estructura en tres áreas clave:
- Saneamiento vegetal in-vitro: protocolos exclusivos para eliminar virus y viroides en cultivos de alto valor y recuperar plantas madre libres de patógenos.
- Genética vegetal: programas de mejora varietal guiados por datos moleculares y trazabilidad genética para certificar variedades y desarrollar nuevas líneas más resistentes y competitivas.
- Consultoría de biotecnología: asesoramiento técnico especializado para integrar soluciones biotecnológicas en la estrategia de productores, viveros y cooperativas.